Types, techniques, preparations and procedures cell staining is a technique used for the main purpose of increasing contrast through changing the color of some of the parts of the structure being observed thus allowing for a clearer view. Easily share your publications and get them in front of issuu’s millions of monthly readers title: bacterial smears and simple stains, author: myriam feldman, name: bacterial smears and simple stains, length: 5 pages, page: 1, published: 2011-07-05 single cells) include the name of the organism if it is not printed and the total magnification discussion: compare the shape and arrangement of the different cultures you stained only two bacterial cultures, but you also observed the. Discussion in this experiment, we focused on gram staining and the mechanisms in gram staining as usual, before we started the experiment, it is crucial for us to perform aseptic technique in order to minimize contamination. Simple stain and the gram stain in most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear (figure 1) in this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to air dry then the slide is heated so that the bacteria are. Lab 2: staining and streaking protocols for simple stain, gram stain, streak plate technique and culture maintenance lab 2a: introduction to staining live specimens are difficult to see with the bright field microscope the contrast between a cell, which is primarily water, and the background, which is water, is poor.
Gram’s staining the gram staining method is named after the danish bacteriologist hans christian gram 2 via a simple anion exchange to produce a chemical precipitate the small chloride anion is replaced by the bulkier iodide, and the complex thus formed becomes insoluble in water during decolorization, alcohol dissolves the lipid present in. The growth was then observed on the agar plate and agar tube in performing the experiment for simple and gram staining, a clean glass slide was obtained, waving the slide over the open flame of a burner a couple times so the remaining dust and stains were removed which is called heat fixation. Using a single stain to color a bacterial cell is commonly referred to as a simple stain the most common dyes for this type of stain are methylene blue, fuchsin, and crystal violet the most common dyes for this type of stain are methylene blue, fuchsin, and crystal violet.
Simple stains provide a quick and easy way to determine cell shape, size, and arrangement 1 perform a bacterial smear, as discussed in figure 3-52 on page 150 of your lab manual. Simple staining in order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide this basically involves 3 steps----transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide. Start studying micro lab 3--preparation of bacterial smears/simple staining/negative staining/gram stain learn vocabulary, terms, and more with flashcards, games, and other study tools. The simple stain can be used to determine cell shape, size, and arrangement true to its name, the simple stain is a very simple staining procedure involving only one stain you may choose from methylene blue, gram safranin, and gram crystal violet.
The gram stain is a type of differential stain that allows a (micro)biologist to identify the differences between organisms and/or differences within the same organism. Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image stains and dyes are frequently used in biology and medicine to highlight structures in. Discussion on simple staining stainin g what is cellular stainingcell staining is a technique that can be used to better visualize cells and cell components under a microscope by using different stains, one can preferentially stain certain cell components, such as a nucleus or a cell wall, or the entire cell.
This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. 2) summarize the fundamental theory of simple staining hint: what scientific property allows the dyes t bind the bacterial cell wall 3) what would happen if you placed a large drop of water on a slide rather than a loopful, when preparing a bacterial smear. Staining microscopic specimens learning objectives differentiate between simple and differential stains describe the unique features of commonly used stains explain the procedures and name clinical applications for gram, endospore, acid-fast, negative capsule, and flagella staining in their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria true to its name, the simple stain is a very simple staining procedure involving single solution of stain. The previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria although simple stains are useful, (1 point) a discussion of the mechanism of gram staining and how it differentiates bacteria on the basis of their cell wall structure should also be included (1 point) the purpose and objectives of the experiment should be stated and a hypothesis should be.
Simple staining: colouration of microorganisms by applying single dye to a fixed smear is termed simple staining one covers the fixed smear with stain for specific period, after which this solution is washed off with water and slide blotted dry. Simple staining technique utilizes single basic dye such as crystal violet, methylene blue, basic fuchsin etc all bacteria take up the basic dye uniformly and appear in the same colour only the morphology of the bacteria can be appreciated upon staining. Discussion a differential staining technique (the schaeffer-fulton method) is used to distinguish between the vegetative cells and the endospores a primary stain (malachite green) is used to stain the endospores grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one. Lab exercise 6: the smear and simple staining objectives 1 prepare bacterial smears for the microscopic visualization of bacteria 2 perform a simple staining procedure.
The simple stain techniqueis a rapid and effective way of preparing a bacterial smear for viewing it is a one-step procedure in which the smear is covered with stain and allowed to sit undisturbed for a minute or so, during which the bacterial cytoplasm chemically unites with the basic. Answer to preparation of a bacteria smear and the simple stain technique 1) identify the steps taken in the preparation of a bact. Simple staining is staining with only one stain/dye there are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Differential and cytological staining techniques theory procedure self evaluation animation assignment reference feedback objective: to study and gain expertise on differential and cytological staining techniques theory: negative capsule staining another simple method to visualize the bacterial capsules is by using negative staining technique.
Although simple stains are useful, they do not reveal details about the bacteria other than morphology and arrangement the gram stain is a differential stain (1 point) a discussion of the mechanism of gram staining and how it differentiates bacteria on the basis of their cell wall structure should also be included. Unformatted text preview: positiveall gram-positive cells are purple in color when stained this week is different then the simple staining lab we did previously because we stained with multiple dyes so it could have came out red or purple rather then automatically purple.